Characterization of methanogenic biomass from a full-scale digester that might harbour SAO activity
نویسندگان
چکیده
Biomass from a full-scale anaerobic digester treating nitrogen-rich wastes was characterized by methanogenic batch activity assays at different ammonia concentrations. SAO was demonstrated by isotopic C fractionation of biogas components, at 3 – 6 gN-TAN. Microbial inhibition was found at 6 gN-TAN both in the acetate consumption rate and by genetic expression studies. Several homoacetogenic eubacteria potentially related to the SAO process were detected by NGS microbiome analysis (5% – 10% in relative abundance), but Methanoculleus spp. was the predominant hydrogenotrophic syntrophic partner (58% of archaeal abundance).
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